About stain results of 450
22:57 28:52 41:05 54:57 39:15 38:48 49:56 47:36 110:08 94:59 77:35 45:29 40:34 112:27 179:44 54:23 51:55 65:10 45:14 59:15 53:07 103:31 68:18 56:46 62:29 148:52 65:56 113:19 113:21 55:08 546:07 95:45 120:08 78:29 94:25 154:10