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  • Microbiology lab safety orientation.

  • After completing this video, you should be able to one,

  • demonstrate proper primary and secondary containment

  • procedures, and two, explain the procedures involved in dealing

  • with a laboratory emergency.

  • Primary containment.

  • Primary containment concerns the protection of personnel

  • and the laboratory environment from exposure

  • to infectious microbes.

  • Proper microbiological techniques,

  • such as the safe transport and disposal of cultures

  • along with the correct use of personal safety equipment,

  • such as gloves and safety goggles

  • go a long way toward accomplishing the goal

  • of primary containment.

  • Secondary containment.

  • Secondary containment deals with protecting

  • the outside environment from exposure

  • to infectious organisms.

  • It depends principally on the design of the laboratory

  • and the availability of equipment, as well,

  • laboratory workers should maintain the lab's safety

  • features, such as keeping a closed door closed

  • or leaving an exhaust fan on.

  • Prior to the lab.

  • Dress appropriately for the lab--

  • no open toed shoes or sandals, and avoid clothing

  • with baggy sleeves that could catch fire

  • or hinder your movement.

  • Know the location of the eyewash, safety shower,

  • fire extinguisher, and first aid kit.

  • Take a moment to learn their operation.

  • During the lab.

  • Always wear a lab coat, gloves, and safety goggles

  • while working in the lab.

  • The lab coat should only be used during lab

  • and should remain in the lab.

  • Even discounting potential biohazards,

  • a lab coat will protect your clothing.

  • There is a reason many of the chemicals you will be

  • working with are called stains.

  • Wash your hands prior to beginning the lab

  • and just before leaving as well.

  • Tie back any long hair, it is both a source of contamination

  • and a fire hazard.

  • Disinfect your benchtop with a disinfectant

  • prior to beginning work and just before leaving the laboratory.

  • Disposal of contaminated materials.

  • Dispose of plastic petri dishes, swabs, disposable gloves,

  • inoculating tools, and similar non-reusable items

  • in the biohazard container.

  • Reusable supplies such as culture test tubes and glass

  • pipettes should have all labels removed

  • before being placed in a rack or container designated

  • for autoclaving.

  • Used microscope slides should be placed

  • in a container for autoclaving or soaked

  • in a disinfectant solution for a minimum of 30 minutes

  • before being discarded.

  • The international biohazard symbol on the containers

  • not only marks the contents for autoclaving prior to disposal,

  • but also cautions anyone in the room

  • as to the possibly hazardous nature of the items

  • inside the containers.

  • And remember, do not overfill the container,

  • and never force objects into the container.

  • Safety considerations.

  • If you are pregnant or if you feel

  • you shouldn't be in the lab because of health concerns,

  • candidly discuss with your instructor.

  • If your skin is exposed to microorganisms

  • as a result of a spill, immediately

  • wash with antiseptic.

  • In the event of a spill, notify your instructor and your lab

  • partners immediately.

  • Broken glass and bacterial cultures

  • are a hazardous combination.

  • With your instructor's approval, cover the spill

  • with paper towels and saturate the towels with disinfectant.

  • After 15 to 20 minutes, carefully wipe up the spill

  • and discard the paper towels in the biohazard container

  • for autoclaving.

  • Discard the broken glass in the sharps container.

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