Name: R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods
Uploaded: 2020-03-29T16:24:26.000Z
Duration: 50 min 42 s
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ELIZABETH NOLAN: What we're going to do today

is just discuss a few aspects of cross linking.

So we decided it was important to introduce

this within recitations this year, because cross-linking

So there's going to be formation of some sort

of covalent linkage between two or maybe more--

more than two reactive groups, OK, of study, right?

So we're chemically joining two or more molecules.

AUDIENCE: Study protein-protein interactions.

And that could be identifying unknown protein-protein

interactions or maybe you know two proteins interact, act

So how might cross-linking help with studying a known

the proteins are actually interacting or which residues

AUDIENCE: It could allow you to isolate them.

So maybe there's an unknown one, and you fish that out,

Or maybe, say, we know that these two proteins interact

So is it on an interface on this side versus maybe

the other side versus maybe behind the board, et cetera.

And really, how I'll present cross-linking today

is in the context of this particular application,

of protein-protein interactions in this course, right?

So just even today, ClpXP is an example, right?

with the ribosome GroEL GroES is an example

And they've been studied by many other methods,

But sometimes maybe it's not possible to get a structure,

And you want to define an interaction surface

or know exactly what residues are important.

But that could be generalized to any other type of molecule,

So can you use cross-linking to learn more

about tertiary structure, quaternary structure?

So imagine for instance, rather than two separate proteins,

we have one protein where there's some flexible linker.

And we have reason to believe these different domains

Again, is it something like this undergoes

like this versus other possibilities here?

So we can capture and identify binding partners,

takes advantage of cross-linking chemistry often.

AUDIENCE: Within the realm of biological things,

I mean, if you want to find a functional root.

So like bioaccumulation or general bioconjugate chemistry

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R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods

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